Import transcript isoform quantification into EZbakRData object
ImportIsoformQuant.Rd
A convenient wrapper to tximport()
for importing isoform quantification
data into an EZbakRData object. This is necessary to run functions such as
EstimateIsoformFractions
.
Usage
ImportIsoformQuant(
obj,
files,
quant_tool = c("none", "salmon", "sailfish", "alevin", "piscem", "kallisto", "rsem",
"stringtie"),
...
)
Arguments
- obj
An
EZbakRData
object.- files
A named vector of paths to all transcript quantification files that you would like to import. This will be passed as the first argument of
tximport::tximport()
(also namedfiles
). The names of this vector should be the same as the sample names as they appear in the metadf of theEZbakRData
object.- quant_tool
String denoting the type of software used to generate the abundances. Will get passed to the
type
argument oftximport::tximport()
. As described in the documentation fortximport
'Options are "salmon", "sailfish", "alevin", "piscem", "kallisto", "rsem", "stringtie", or "none". This argument is used to autofill the arguments below (geneIdCol, etc.) "none" means that the user will specify these columns. Be aware that specifying type other than "none" will ignore the arguments below (geneIdCol, etc.)'. Referenced 'arguments below' can be specified as part of...
.- ...
Additional arguments to be passed to
tximport::tximport()
. Especially relevant if you setquant_tool
to "none".